It is another interdisciplinary region including organic, measurable and computational sciences. Now a days bioinformatics innovation is for the most part utilized for the special growth investigation. Ribosomal S6 protein kinase 4 (RSK4) is a vital novel tumor silencer that hinders bosom disease cell development and initiates senescence. Utilizing pair proclivity cleaning, we got protein buildings that associated with RSK4 or RSK4m. Mass range examination was performed to distinguish the acquired protein edifices, and bioinformatics investigation was performed.